Preparation of bone marrow cells-derived extracellular matrix and its microstructure and composition

doi:10.3969/j.issn.2095-4344.2014.10.024

Abstract

Abstract:

BACKGROUND: Extracellular matrix can simulate microenvironment and make the stem cells proliferate maintaining the characteristics of stem cells well in vitro.
OBJECTIVE: To prepare the extracellular matrix from human bone marrow cells and to analyze its microstructure and composition preliminarily.
METHODS: Human bone marrow cells of passage 4 were cultured for 14 days, and the induction medium was used during the last 8 days. After decellularization, cells were removed to prepare human bone marrow cells-derived extracellular matrix. The surface morphology of human bone marrow cells-derived extracellular matrix was observed by inverted microscope and scanning electron microscope. Changes of collagen I and biglycan before and after decellularization were observed by immunofluorescence staining. Human periodontal ligament stem cells were seeded onto human bone marrow cells-derived extracellular matrix, fibronectin coated 6-well plate and normal culture plate to compare the influence of different matrix on cell morphology and adhesion.
RESULTS AND CONCLUSION: We obtained intact human bone marrow cells-derived extracellular matrix by chemical combined with physical decellularization. The structure and amount of collagen I and biglycan had not been compromised dramatically after decellularization. Human periodontal ligament stem cells growing on the human bone marrow cells-derived extracellular matrix developed in accordance with the orbit of the extracellular matrix, differing from the original cell morphology. There were more human periodontal ligament stem cells adhering to the extracellular matrix during the same time. These findings indicate that effective decellularization can produce intact the extracellular matrix membrane without destroying its microstructure. Extracellular matrix protein is not compromised due to decellularization. The extracellular matrix affects cell morphology and promotes cell adhesion. We can use the extracellular matrix model to simulate stem cell microenvironment and thereafter, acquire a large number of adult stem cells with high quality in vitro.

中国组织工程研究杂志出版内容重点：干细胞；骨髓干细胞；造血干细胞；脂肪干细胞；肿瘤干细胞；胚胎干细胞；脐带脐血干细胞；干细胞诱导；干细胞分化；组织工程

全文链接：

Key words: stem cells, extracellular matrix, bone marrow cells

CLC Number:

R394.2

Tang Li, Wu Jun-jie, Wang A-xian, Liang Yuan, Ji Hai-ning, Ding Yin . Preparation of bone marrow cells-derived extracellular matrix and its microstructure and composition[J]. Chinese Journal of Tissue Engineering Research, 2014, 18(10): 1629-1634.

Figures/Tables 4

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